Publication in the Diário da República: Despacho nº 10764/2011 - 30/08/2011
5.5 ECTS; 3º Ano, 1º Semestre, 30,0 T + 30,0 P , Cód. 918431.
Lecturer
(1) Docente Responsável
(2) Docente que lecciona
Prerequisites
Knowledge of biochemistry and cellular biology.
Objectives
The students must acquire knowledge in the area of genetic engineering, through the learning of the fundamental concepts of
molecular biology, genetics, and the applications of recombinant DNA technology. They should gain expertise in the use of relevant molecular techniques.
Program
DNA structure, replication, restriction, repair and recombination. Transcription. Genetic code and translation. Protein synthesis. Regulation of genetic expression. Recombinant DNA technology. Restriction enzymes and cloning vectors. Instability of r-plasmids. PCR, Southern Blot, Foot-printing,Northern Blot, basis and applications. Sequencing of DNA fragments. Genomic banks. Bioinformatics.
Evaluation Methodology
Final written test, presentation and discussion of a practical assignment and laboratory reports.
Bibliography
- Rehm, H. (1993). Biotechnology Genetic Fundamentals and Genetic Engineering. (Vol. 2). New York: VCH Publishers INC
- Videira, A. (2001). Engenharia Genética Princípios e Aplicações (Princípios básicos - Cap I a VIII), . Lisboa: Lidel-Edições Técnicas
- Mateus, D. (0). Sebenta de Engenharia Genética. Acedido em 2 de fevereiro de 2012 em www.e-learning.ipt.pt
- Mota, M. e Lima, N. (2003). Biotecnologia Fundamentos e Aplicações (Genética aplicada- Cap VI e VII). Lisboa: Lidel-Edições Técnicas
Teaching Method
Theoretical lectures, tutorials and laboratory classes.
Software used in class
