Publication in the Diário da República: NI n.º1417|EAB|ESTT|2011
5.5 ECTS; 3º Ano, 1º Semestre, 30,0 T + 30,0 P , Cód. 910032.
Lecturer
(1) Docente Responsável
(2) Docente que lecciona
Prerequisites
Concepts of biochemistry and cellular biology.
Objectives
The students must acquire knowledge in the area of genetic engineering by learning the fundamental concepts of
molecular biology, genetics, and the applications of recombinant
DNA technology.
Program
DNA structure, replication, restriction, repair and recombination. Transcription. Genetic code and translation. Protein synthesis. Regulation of
genetic expression. Recombinant DNA technology. Restriction enzymes and cloning vectors. Instability of r-plasmids. PCR, Southern Blot, Foot-printing, Northern Blot, basis and applications. Sequencing of DNA fragments. Genomic banks. Bioinformatics.
Evaluation Methodology
Final written test, presentation and discussion of a practical assignment and reports on laboratorial activities.
Bibliography
- Rehm, H. (1993). Biotechnology Genetic Fundamentals and Genetic Engineering. (Vol. 2). New York: VCH Publishers INC
- Videira, A. (2001). Engenharia Genética Princípios e Aplicações (Princípios básicos - Cap I a VIII), . Lisboa: Lidel-Edições Técnicas
- Mateus, D. (0). Sebenta de Engenharia Genética. Acedido em 2 de fevereiro de 2012 em www.e-learning.ipt.pt
- Mota, M. e Lima, N. (2003). Biotecnologia Fundamentos e Aplicações (Genética aplicada- Cap VI e VII). Lisboa: Lidel-Edições Técnicas
Teaching Method
Lectures and laboratory sessions including problem solving.
Software used in class
